Course Content:
UNIT –I 
UV Visible spectroscopy

  • Electronic transitions
  • Chromophores and Auxochromes
  • Spectral shifts,
  • Solvent effect on absorption spectra
  • Beer and Lambert’s law -Derivation and deviations

Instrumentation –

  • Sources of radiation,
  • Wavelength selectors,
  • Sample cells
  • Detectors- Photo tube, Photomultiplier tube, Photovoltaic cell, Silicon Photodiode.

Applications – Spectrophotometric titrations, Single-component and multi-component analysis

Fluorimetry

  • Theory
  • Concepts of singlet, doublet and triplet electronic states
  • Internal and external conversions
  • Factors affecting fluorescence
  • Quenching
  • Instrumentation and Applications

UNIT –II 
IR spectroscopy

  • Introduction
  • Fundamental modes of vibrations in polyatomic molecules
  • Sample handling,
  • Factors affecting vibrations

Instrumentation –

  • Sources of radiation,
  • wavelength selectors,
  • Detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector

Applications

Flame Photometry

  • Principle
  • Interferences,
  • Instrumentation and Applications

Atomic absorption spectroscopy

  • Principle
  • Interferences
  • Instrumentation and Applications

Nepheloturbidometry

  • Principle
  • Instrumentation and Applications

UNIT –III 
Introduction to chromatography

  • Adsorption and partition column chromatography
  • Methodology
  • Advantages and Disadvantages
  • Applications

Thin-layer chromatography

  • Introduction
  • Principle
  • Methodology
  • Rf values
  • Advantages and Disadvantages
  • Applications

Paper Chromatography

  • Introduction
  • Methodology
  • Development techniques
  • Advantages and Disadvantages
  • Applications

Electrophoresis

  • Introduction
  • Factors affecting electrophoretic mobility
  • Techniques of paper, gel, capillary electrophoresis,
  • Applications

UNIT-IV 
Gas Chromatography 

  • Introduction
  • Theory
  • Instrumentation,
  • Derivatization
  • Temperature programming,
  • Advantages and disadvantages
  • Applications

High-performance liquid chromatography (HPLC)

  • Introduction,
  • Theory
  • Instrumentation
  • Advantages and applications.

UNIT –V
Ion exchange chromatography

  • Introduction,
  • Classification
  • Ion exchange resins
  • Properties
  • Mechanism of the ion exchange process,
  • Factors affecting ion exchange,
  • Methodology and Applications

Gel Chromatography

  • Introduction
  • Theory
  • Instrumentation and Applications

Affinity chromatography

  • Introduction
  • Theory
  • Instrumentation and Applications

About Instructor

Inpharmedia

Inpharmedia is a platform to equip the aspirants with academic excellence, domain expertise that delivers neXZen Professional to make graduates future ready.

24 Courses

Not Enrolled
Rs 149/-

Course Includes

  • 7 Lessons
  • 6 Topics
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